Definition: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Other names n-Hybrid System Technique; n Hybrid System Techniques; Yeast Two-Hybrid Systems; Yeast Two-Hybrid Assays; Yeast Two Hybrid System Techniques; Yeast Two Hybrid System; Yeast Two Hybrid Assay; Yeast Three-Hybrid Systems; Yeast Three-Hybrid Assays; Yeast Three Hybrid System Techniques; Yeast Three Hybrid System; Yeast Three Hybrid Assay; Yeast One Hybrid System Techniques; Two-Hybrid Systems, Yeast; Two-Hybrid System, Yeast; Two-Hybrid System Technique; Two-Hybrid System Technic; Two-Hybrid Methods; Two-Hybrid Assays, Yeast; Two-Hybrid Assays
