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Dithionitrobenzoic Acid

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Definition: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.     
Pharmacological Action Sulfhydryl Reagents 		Other names Ellman's Reagent; DTNB; 5,5'-Dithiobis(2-nitrobenzoic Acid); Reagent, Ellman's; Ellmans Reagent; Ellman Reagent; Acid, Dithionitrobenzoic; 5,5'-Dithiobis(nitrobenzoate)
 
SubstanceCAS Registry & nameCategoriesSourceDrugs*
nonylene-1,9-bis(5-dithio-2-nitrobenzoic acid)  142093-99-0   Dithionitrobenzoic Acid/*analogs & derivatives. FEBS Lett 1992 May 18;302(3):201-5
6,6'-diselenobis-(3-nitrobenzoic acid)  80180-68-3 Benzoic acid, 2,2'-diselenobis(5-nitro)  Dithionitrobenzoic Acid/*analogs & derivatives Sulfhydryl Reagents. Anal Biochem 1981;117(1):94

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Sources: NLM Medical Subject Headings, NIH UMLS, Drugs@FDA, FDA AERS original data copyright United States Government. No endorsement implied. Last modified 6/6/2012
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